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Archive for March 4th, 2011


Dr Matthias Langhorst from Carl Zeiss MicroImaging in Göttingen, Germany, will be presenting a seminar on advanced light microscopy.
Mon, March 7, 4pm – 5pm
SR2, Life Sciences Building

How to expand the current boundaries of light microscopy?

The last decades have seen a tremendous development of the performance of light microscopes in the life sciences. The dynamics of single molecules in living cells can now be imaged at high frame rates, cellular behaviour in development can be followed over days and subcellular details can be captured at a resolution surpassing the classical diffraction limit. However, there are still some very fundamental limits to the performance of a light microscope.

Observation of living samples is still ultimately limited by phototoxic side effects. Whenever the goal is to image deeper into thicker specimens, it is still a big challenge to achieve sufficient resolution and contrast. As we move from pure images to statistically convincing data, collecting images at sufficient contrast for automatic analysis proves to be difficult. In this talk, I will provide some examples of promising ways to overcome the current limitations of light microscopy. New approaches to labeling, active and dynamic illumination and detection schemes as well as new instrument geometries will completely change the ways of microscopy. The next decades will witness a revolution, with fundamentally new set-ups and experimental protocols providing new levels of imaging performance in life science research.

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